Intraceilular distribution of H+-ATPase and Cl--ATPase in rat brain
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چکیده
منابع مشابه
Proteolytic digestion of Cl(-)-ATPase in rat brain synaptosomes.
Upon tryptic digestion of synaptosomes, ATPase activities decreased in the order of Cl(-)-ATPase greater than or equal to Na+,K+-ATPase greater than anion-insensitive Mg2+-ATPase. Upon synaptosome treatment with hypotonic solution, Cl(-)-ATPase or anion-insensitive Mg2+-ATPase was slightly inactivated, while Na+,K+-ATPase underwent a much larger degree of inactivation. ATP-Mg inhibited the ATPa...
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To explore the action mechanism of lithium in the brain, the author investigated the effects of lithium on Na-K ATPase and Ca ATPase in rat brain synaptosomes prepared from forebrains by the method of Booth and Clark. The activities of Na-K ATPase and Ca ATPase were assayed by the level of inorganic phosphate liberated from the hydrolysis of ATP. Lithium at the optimum therapeutic concentration...
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The aim of the work was to investigate the interaction between borate capped gold nanoparticles (NPs) and the rat brain synaptosomal plasma membranes (SPM), as well as the effects of these NPs on SPM Na/KATPase and Mg-ATPase activity. The changes in the UV-vis spectra of NPs and SPM assembly suggested the agglomeration and precipitation of NPs. FTIR measurements indicated that both protein –SH ...
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It has been determined that Mg2+ stimulates phosphorylation while Cl- stimulates dephosphorylation of the chloride pump during its reaction sequence. The stoichiometry of ATP hydrolyzed to Cl- transported during a single cycle of the reaction sequence was ascertained. Intracellular concentrations of ATP, ADP and inorganic phosphate were determined and, coupled with an estimate of the standard f...
متن کاملHeavy metal regulation of plasma membrane H+-ATPase gene expression in halophyte Aeluropus littoralis
The present study was conducted to find the effect of three heavy metals, Ag, Hg and Pb on the expression level of a gene encoding plasma membrane H+-ATPase in Aeluropus littoralis. The experiment was laid out in a completely random design with three replications. The expression of the main gene was normalized to the expression of the housekeeping gene actin. Two 259 and 187 bp fragments were a...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1986
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)59476-8